KIT Kit CLIA

N° du produit ABIN5658954

Détail du produit KIT Kit CLIA

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 468.75 pg/mL - 30000 pg/mL

Seuil minimal de détection: 468.75 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor Receptor (SCFR). No significant cross-reactivity or interference between Stem Cell Factor Receptor (SCFR) and analogues was observed.

Sensibilité: 15.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor (SCFR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor Receptor (SCFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor Receptor (SCFR) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor Receptor (SCFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Autre désignation: Stem Cell Factor Receptor (KIT Produits)

Synonymes: C-Kit Kit CLIA, CD117 Kit CLIA, PBT Kit CLIA, SCFR Kit CLIA, Bs Kit CLIA, Fdc Kit CLIA, Gsfsco1 Kit CLIA, Gsfsco5 Kit CLIA, Gsfsow3 Kit CLIA, SCO1 Kit CLIA, SCO5 Kit CLIA, SOW3 Kit CLIA, Ssm Kit CLIA, Tr-kit Kit CLIA, W Kit CLIA, c-KIT Kit CLIA, c-kit Kit CLIA, MGF Kit CLIA, KIT Kit CLIA, C-KIT Kit CLIA, kit Kit CLIA, receptor Kit CLIA, sparse Kit CLIA, Xkl-1 Kit CLIA, kit-a Kit CLIA, kit-b Kit CLIA, kl1-A Kit CLIA, krk1 Kit CLIA, pbt Kit CLIA, scfr Kit CLIA, KIT proto-oncogene receptor tyrosine kinase Kit CLIA, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Kit CLIA, KIT proto-oncogene receptor tyrosine kinase S homeolog Kit CLIA, KIT Kit CLIA, Kit Kit CLIA, kita Kit CLIA, kit Kit CLIA, kit.S Kit CLIA

Sujet:

Gene Name: Stem Cell Factor Receptor

Gene Aliases: CD117, KIT, C-Kit, PBT, p145 c-Kit, C-Kit Receptor, Tyrosine-Protein Kinase, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog, Piebald Trait

ID gène: 3815

UniProt: P10721

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity