SRD5A2 Kit ELISA

N° du produit ABIN5658960

Détail du produit SRD5A2 Kit ELISA

Antigène: SRD5A2 (Steroid-5-alpha-Reductase, alpha Polypeptide 2 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 2) (SRD5A2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 2 (SRD5a2). No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 2 (SRD5a2) and analogues was observed.

Sensibilité: 0.062 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 2 (SRD5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Steroid 5 Alpha Reductase 2 (SRD5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SRD5A2 (Steroid-5-alpha-Reductase, alpha Polypeptide 2 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 2) (SRD5A2))

Autre désignation: Steroid 5 Alpha Reductase 2 (SRD5A2 Produits)

Synonymes: S5AR 2 Kit ELISA, 5ART2 Kit ELISA, SRD5alpha2 Kit ELISA, SRD5A2 Kit ELISA, Srd5a2 Kit ELISA, CH73-233A22.6 Kit ELISA, wu:fk70d02 Kit ELISA, zgc:109942 Kit ELISA, zgc:112208 Kit ELISA, zgc:112455 Kit ELISA, steroid 5 alpha-reductase 2 Kit ELISA, steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) Kit ELISA, 3-oxo-5-alpha-steroid 4-dehydrogenase 2 Kit ELISA, steroid-5-alpha-reductase, alpha polypeptide 2a Kit ELISA, steroid-5-alpha-reductase, alpha polypeptide 2b Kit ELISA, SRD5A2 Kit ELISA, Srd5a2 Kit ELISA, srd5a2 Kit ELISA, LOC100125532 Kit ELISA, srd5a2a Kit ELISA, srd5a2b Kit ELISA

Sujet:

Gene Name: Steroid 5 Alpha Reductase 2

Gene Aliases: 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 2

ID gène: 94224

UniProt: Q99N99

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis