TCIRG1 Kit ELISA

N° du produit ABIN5659081

Détail du produit TCIRG1 Kit ELISA

Antigène: TCIRG1 (T-Cell, Immune Regulator 1, ATPase, H+ Transporting, Lysosomal V0 Subunit A3 (TCIRG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of T-Cell, Immune Regulator 1 (TCIRG1). No significant cross-reactivity or interference between T-Cell, Immune Regulator 1 (TCIRG1) and analogues was observed.

Sensibilité: 6.0 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell, Immune Regulator 1 (TCIRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell, Immune Regulator 1 (TCIRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TCIRG1 (T-Cell, Immune Regulator 1, ATPase, H+ Transporting, Lysosomal V0 Subunit A3 (TCIRG1))

Autre désignation: T-Cell, Immune Regulator 1 (TCIRG1 Produits)

Synonymes: OC116 Kit ELISA, TIRC7 Kit ELISA, ATP6N1C Kit ELISA, ATP6a3 Kit ELISA, Atp6i Kit ELISA, OC-116 Kit ELISA, OPTB1 Kit ELISA, Stv1 Kit ELISA, Vph1 Kit ELISA, oc Kit ELISA, ATP6V0A3 Kit ELISA, OC-116kDa Kit ELISA, a3 Kit ELISA, T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 Kit ELISA, T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 Kit ELISA, T-cell immune regulator 1, ATPase H+ transporting V0 subunit a3 Kit ELISA, Tcirg1 Kit ELISA, TCIRG1 Kit ELISA

Sujet:

Gene Name: T-Cell, Immune Regulator 1

Gene Aliases: Stv1, Vph1, ATP6N1C, ATP6V0A3, Atp6i, OC-116 kDa, OC116, OPTB1, TIRC7, a3, V-Type Proton ATPase 116 kDa Subunit A Isoform 3, Osteoclastic proton pump 116 kDa subunit

Pathways: Transition Metal Ion Homeostasis, Proton Transport