TAF13 Kit ELISA

N° du produit ABIN5659097

Détail du produit TAF13 Kit ELISA

Antigène: TAF13 (TAF13 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 18kDa (TAF13))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of TATA Box Binding Protein Associated Factor 13 (TAF13). No significant cross-reactivity or interference between TATA Box Binding Protein Associated Factor 13 (TAF13) and analogues was observed.

Sensibilité: 0.128 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TATA Box Binding Protein Associated Factor 13 (TAF13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TATA Box Binding Protein Associated Factor 13 (TAF13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TATA Box Binding Protein Associated Factor 13 (TAF13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TATA Box Binding Protein Associated Factor 13 (TAF13) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TATA Box Binding Protein Associated Factor 13 (TAF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TAF13 (TAF13 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 18kDa (TAF13))

Autre désignation: TATA Box Binding Protein Associated Factor 13 (TAF13 Produits)

Synonymes: TAF(II)18 Kit ELISA, TAF2K Kit ELISA, TAFII-18 Kit ELISA, TAFII18 Kit ELISA, wu:fc20d03 Kit ELISA, zgc:103651 Kit ELISA, 1810004N01Rik Kit ELISA, 2010309N11Rik Kit ELISA, AI847295 Kit ELISA, TATA-box binding protein associated factor 13 Kit ELISA, TATA-box binding protein associated factor 13 S homeolog Kit ELISA, TAF13 Kit ELISA, taf13 Kit ELISA, taf13.S Kit ELISA, Taf13 Kit ELISA

Sujet:

Gene Name: TATA Box Binding Protein Associated Factor 13

Gene Aliases: TAF2K, TAFII18, Transcription Initiation Factor TFIID Subunit 13, Transcription initiation factor TFIID 18 kDa subunit

ID gène: 6884

UniProt: Q15543