TJP1 Kit ELISA

N° du produit ABIN5659256

Détail du produit TJP1 Kit ELISA

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tight Junction Protein 1 (TJP1). No significant cross-reactivity or interference between Tight Junction Protein 1 (TJP1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Protein 1 (TJP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tight Junction Protein 1 (TJP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tight Junction Protein 1 (TJP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tight Junction Protein 1 (TJP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tight Junction Protein 1 (TJP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tight Junction Protein 1 (TJP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Autre désignation: Tight Junction Protein 1 (TJP1 Produits)

Synonymes: ZO-1 Kit ELISA, ZO1 Kit ELISA, zo1 Kit ELISA, tight junction protein 1 Kit ELISA, tight junction protein ZO-1 Kit ELISA, TJP1 Kit ELISA, Tjp1 Kit ELISA, LOC396567 Kit ELISA

Sujet:

Gene Name: Tight Junction Protein 1

Gene Aliases: ZO1, Zona Occludens 1, Zona occludens protein 1, Zonula occludens protein 1

ID gène: 292994

UniProt: F1M4A0

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization