TIRAP Kit ELISA

N° du produit ABIN5659346

Détail du produit TIRAP Kit ELISA

Antigène: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP). No significant cross-reactivity or interference between Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) and analogues was observed.

Sensibilité: 0.109 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TIRAP (Toll-Interleukin 1 Receptor (TIR) Domain Containing Adaptor Protein (TIRAP))

Autre désignation: Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP Produits)

Synonymes: mal Kit ELISA, wyatt Kit ELISA, BACTS1 Kit ELISA, Mal Kit ELISA, MyD88-2 Kit ELISA, AA407980 Kit ELISA, C130027E04Rik Kit ELISA, Tlr4ap Kit ELISA, Wyatt Kit ELISA, toll-interleukin 1 receptor (TIR) domain containing adaptor protein Kit ELISA, TIR domain containing adaptor protein Kit ELISA, toll-interleukin 1 receptor (TIR) domain containing adaptor protein L homeolog Kit ELISA, toll-interleukin 1 receptor (TIR) domain-containing adaptor protein Kit ELISA, tirap Kit ELISA, TIRAP Kit ELISA, tirap.L Kit ELISA, Tirap Kit ELISA

Sujet:

Gene Name: Toll Interleukin 1 Receptor Domain Containing Adaptor Protein

Gene Aliases: Mal, Wyatt, MyD88 Adapter-Like, Adaptor protein Wyatt

ID gène: 114609

UniProt: P58753

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades