TLR4 Kit CLIA

N° du produit ABIN5659358

Détail du produit TLR4 Kit CLIA

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 39.06 pg/mL - 10000 pg/mL

Seuil minimal de détection: 39.06 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 4 (TLR4). No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.

Sensibilité: 5.83 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 4 (TLR4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Toll Like Receptor 4 (TLR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Toll Like Receptor 4 (TLR4) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TLR4 (Toll-Like Receptor 4 (TLR4))

Autre désignation: Toll Like Receptor 4 (TLR4 Produits)

Synonymes: ARMD10 Kit CLIA, CD284 Kit CLIA, TLR-4 Kit CLIA, TOLL Kit CLIA, Lps Kit CLIA, Ly87 Kit CLIA, Ran/M1 Kit CLIA, Rasl2-8 Kit CLIA, toll like receptor 4 Kit CLIA, toll-like receptor 4 Kit CLIA, TLR4 Kit CLIA, Tlr4 Kit CLIA

Sujet:

Gene Name: Toll Like Receptor 4

Gene Aliases: CD284, TOLL, HToll

ID gène: 21898

UniProt: Q9QUK6

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins