Transferrin Kit CLIA

N° du produit ABIN5659406

Détail du produit Transferrin Kit CLIA

Antigène: Transferrin (TF)

Reactivité: Rat

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 40000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transferrin (TRF). No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.

Sensibilité: 20.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transferrin (TRF) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TRF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TRF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Transferrin (TF)

Autre désignation: Transferrin (TF Produits)

Synonymes: ltf Kit CLIA, pro1557 Kit CLIA, pro2086 Kit CLIA, mgc107777 Kit CLIA, LOC692564 Kit CLIA, TF Kit CLIA, LOC100144362 Kit CLIA, tf Kit CLIA, LTF Kit CLIA, TFEW Kit CLIA, conalbumin Kit CLIA, tf-b Kit CLIA, MGC64306 Kit CLIA, PRO1557 Kit CLIA, PRO2086 Kit CLIA, TFQTL1 Kit CLIA, AI266983 Kit CLIA, Cd176 Kit CLIA, HP Kit CLIA, Tf Kit CLIA, Tfn Kit CLIA, hpx Kit CLIA, Trf Kit CLIA, cb285 Kit CLIA, gavi Kit CLIA, id:ibd3238 Kit CLIA, id:ibd3525 Kit CLIA, sb:cb285 Kit CLIA, wu:fb57g06 Kit CLIA, wu:fb62h02 Kit CLIA, wu:fb63h10 Kit CLIA, wu:fb64h10 Kit CLIA, zgc:112154 Kit CLIA, 143958_at Kit CLIA, CG6186 Kit CLIA, Dmel\\CG6186 Kit CLIA, TSF1 Kit CLIA, anon-EST:Posey265 Kit CLIA, tsf1 Kit CLIA, Pro-TRH Kit CLIA, IL-5 Kit CLIA, STF I Kit CLIA, TRF1 Kit CLIA, sTF1 Kit CLIA, sTf Kit CLIA, tf1 Kit CLIA, transferrin Kit CLIA, serotransferrin Kit CLIA, transferrin (ovotransferrin) Kit CLIA, transferrin L homeolog Kit CLIA, melanotransferrin Kit CLIA, transferrin-a Kit CLIA, Transferrin 1 Kit CLIA, thyrotropin releasing hormone Kit CLIA, interleukin 5 Kit CLIA, TF Kit CLIA, LOC477072 Kit CLIA, tf Kit CLIA, Tf Kit CLIA, LOC100144362 Kit CLIA, tf.L Kit CLIA, LOC5575625 Kit CLIA, Trf Kit CLIA, tfa Kit CLIA, Tsf1 Kit CLIA, TRH Kit CLIA, IL5 Kit CLIA, LOC101085148 Kit CLIA, LOC100726872 Kit CLIA, trf Kit CLIA

Sujet:

Gene Name: Transferrin

Gene Aliases: TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin

UniProt: P12346

Pathways: Transition Metal Ion Homeostasis