TGFB1 Kit CLIA

N° du produit ABIN5659432

Détail du produit TGFB1 Kit CLIA

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivité: Porc

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1). No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.

Sensibilité: 5.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Beta 1 (TGFb1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: Transforming Growth Factor Beta 1 (TGFB1 Produits)

Synonymes: CED Kit CLIA, DPD1 Kit CLIA, LAP Kit CLIA, TGFB Kit CLIA, TGFbeta Kit CLIA, TGF-beta Kit CLIA, TGF-BETA-1 Kit CLIA, TGF-beta5 Kit CLIA, ced Kit CLIA, dpd1 Kit CLIA, lap Kit CLIA, tgf-beta Kit CLIA, tgfb Kit CLIA, tgfb5 Kit CLIA, tgfbeta Kit CLIA, TGF-beta1 Kit CLIA, TGFbeta1 Kit CLIA, Tgfb Kit CLIA, Tgfb-1 Kit CLIA, ai39657 Kit CLIA, tgfb1 Kit CLIA, wu:fb13a07 Kit CLIA, xx:ai39657 Kit CLIA, TGFB1 Kit CLIA, csd Kit CLIA, cdb1 Kit CLIA, cdg2 Kit CLIA, csd1 Kit CLIA, csd2 Kit CLIA, csd3 Kit CLIA, ebmd Kit CLIA, lcd1 Kit CLIA, bigh3 Kit CLIA, cdgg1 Kit CLIA, betaig-h3 Kit CLIA, TGFB4 Kit CLIA, transforming growth factor beta 1 Kit CLIA, transforming growth factor beta-1 Kit CLIA, transforming growth factor beta 1 L homeolog Kit CLIA, transforming growth factor, beta 1 Kit CLIA, transforming growth factor, beta 1a Kit CLIA, transforming growth factor beta induced L homeolog Kit CLIA, TGFB1 Kit CLIA, Tgfb1 Kit CLIA, tgfb1.L Kit CLIA, tgfb1a Kit CLIA, tgfbi.L Kit CLIA

Sujet:

Gene Name: Transforming Growth Factor Beta 1

Gene Aliases: TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide

ID gène: 397078

UniProt: P07200

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints