TNFRSF10B Kit ELISA

N° du produit ABIN5659663

Détail du produit TNFRSF10B Kit ELISA

Antigène: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) and analogues was observed.

Sensibilité: 0.062 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Autre désignation: Tumor Necrosis Factor Receptor Superfamily, Member 10B (TNFRSF10B Produits)

Synonymes: TNFRSF10B Kit ELISA, dr5 Kit ELISA, cd262 Kit ELISA, killer Kit ELISA, trick2 Kit ELISA, trickb Kit ELISA, xDR-M1 Kit ELISA, xDR-M2 Kit ELISA, ztnfr9 Kit ELISA, trailr2 Kit ELISA, trick2a Kit ELISA, trick2b Kit ELISA, CD262 Kit ELISA, DR5 Kit ELISA, KILLER Kit ELISA, KILLER/DR5 Kit ELISA, TRAIL-R2 Kit ELISA, TRAILR2 Kit ELISA, TRICK2 Kit ELISA, TRICK2A Kit ELISA, TRICK2B Kit ELISA, TRICKB Kit ELISA, ZTNFR9 Kit ELISA, Ly98 Kit ELISA, MK Kit ELISA, tumor necrosis factor receptor superfamily, member 10b Kit ELISA, tumor necrosis factor receptor superfamily member 10b S homeolog Kit ELISA, tumor necrosis factor receptor superfamily member 10b L homeolog Kit ELISA, tumor necrosis factor receptor superfamily member 10b Kit ELISA, TNF receptor superfamily member 10b Kit ELISA, Tnfrsf10b Kit ELISA, TNFRSF10B Kit ELISA, tnfrsf10b.S Kit ELISA, tnfrsf10b.L Kit ELISA, tnfrsf10b Kit ELISA

Sujet:

Gene Name: Tumor Necrosis Factor Receptor Superfamily, Member 10B

Gene Aliases: CD262, DR5, KILLER, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2

Pathways: Signalisation p53, Apoptose, Positive Regulation of Endopeptidase Activity