TNFRSF1B Kit ELISA

N° du produit ABIN5659672

Détail du produit TNFRSF1B Kit ELISA

Antigène: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Autre désignation: Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B Produits)

Synonymes: CD120b Kit ELISA, TBPII Kit ELISA, TNF-R-II Kit ELISA, TNF-R75 Kit ELISA, TNFBR Kit ELISA, TNFR1B Kit ELISA, TNFR2 Kit ELISA, TNFR80 Kit ELISA, p75 Kit ELISA, p75TNFR Kit ELISA, TNF-RII/TNFRSF1B Kit ELISA, TNFR-II Kit ELISA, TNFRSF1B Kit ELISA, TNF-R2 Kit ELISA, TNF-alphaR2 Kit ELISA, TNFRII Kit ELISA, TNFalpha-R2 Kit ELISA, Tnfr-1 Kit ELISA, Tnfr2 Kit ELISA, rank Kit ELISA, zgc:163064 Kit ELISA, TNF receptor superfamily member 1B Kit ELISA, tumor necrosis factor receptor superfamily, member 1b Kit ELISA, tumor necrosis factor receptor superfamily, member 1B Kit ELISA, TNFRSF1B Kit ELISA, Tnfrsf1b Kit ELISA, tnfrsf1b Kit ELISA

Sujet:

Gene Name: Tumor Necrosis Factor Receptor Superfamily, Member 1B

Gene Aliases: CD120B, P75, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80, P75tnfr, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, Etanercept

ID gène: 7133

UniProt: P20333

Pathways: Signalisation NF-kappaB, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway