LMYC Kit ELISA

N° du produit ABIN5659812

Détail du produit LMYC Kit ELISA

Antigène: LMYC (MYCL) (Protein L-Myc (MYCL))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). No significant cross-reactivity or interference between V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) and analogues was observed.

Sensibilité: 0.59 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LMYC (MYCL) (Protein L-Myc (MYCL))

Autre désignation: V-Myc Myelocytomatosis Viral Oncogene Homolog (MYCL Produits)

Synonymes: MRTL Kit ELISA, MYCC Kit ELISA, bHLHe39 Kit ELISA, c-Myc Kit ELISA, MYCL1 Kit ELISA, Lmyc1 Kit ELISA, Mycl1 Kit ELISA, LMYC Kit ELISA, bHLHe38 Kit ELISA, CMYC Kit ELISA, c-myc Kit ELISA, ARC Kit ELISA, B430311C09Rik Kit ELISA, MYC Kit ELISA, NOP Kit ELISA, Nop30 Kit ELISA, RNCMYC Kit ELISA, mMyc Kit ELISA, C-MYC Kit ELISA, AU016757 Kit ELISA, Myc2 Kit ELISA, Niard Kit ELISA, Nird Kit ELISA, AW536278 Kit ELISA, L-myc Kit ELISA, Lmyc-1 Kit ELISA, l-myc Kit ELISA, lmyc Kit ELISA, lmyc-b Kit ELISA, lmyc2 Kit ELISA, mycl1-b Kit ELISA, xL-Myc2 Kit ELISA, fi04c04 Kit ELISA, mycl1b Kit ELISA, si:ch211-194e15.2 Kit ELISA, wu:fa07c04 Kit ELISA, wu:fb19f03 Kit ELISA, wu:fi04c04 Kit ELISA, zgc:152988 Kit ELISA, lmyc1 Kit ELISA, mycl1 Kit ELISA, mycl1a Kit ELISA, zgc:85967 Kit ELISA, lmyc-A Kit ELISA, mycl Kit ELISA, mycl1-a Kit ELISA, xL-Myc1 Kit ELISA, xL-myc1 Kit ELISA, MYC proto-oncogene, bHLH transcription factor Kit ELISA, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived homolog Kit ELISA, MYCL proto-oncogene, bHLH transcription factor Kit ELISA, nucleolar protein 3 (apoptosis repressor with CARD domain) Kit ELISA, myelocytomatosis oncogene Kit ELISA, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived Kit ELISA, MYCL proto-oncogene, bHLH transcription factor S homeolog Kit ELISA, MYCL proto-oncogene, bHLH transcription factor b Kit ELISA, MYCL proto-oncogene, bHLH transcription factor a Kit ELISA, MYCL proto-oncogene, bHLH transcription factor L homeolog Kit ELISA, MYC Kit ELISA, MYCL Kit ELISA, Mycl Kit ELISA, Nol3 Kit ELISA, Myc Kit ELISA, mycl.S Kit ELISA, myclb Kit ELISA, mycla Kit ELISA, mycl.L Kit ELISA

Sujet:

Gene Name: V-Myc Myelocytomatosis Viral Oncogene Homolog

Gene Aliases: C-Myc, BHLHE39, Myc Proto-Oncogene Protein, Class E basic helix-loop-helix protein 39, Transcription factor p64, Proto-oncogene c-Myc

ID gène: 4609

UniProt: P01106

Pathways: L'effet Warburg