Versican Kit CLIA

N° du produit ABIN5659918

Détail du produit Versican Kit CLIA

Antigène: Versican (Vcan)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Versican (VS). No significant cross-reactivity or interference between Versican (VS) and analogues was observed.

Sensibilité: 12.59 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Versican (VS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Versican (VS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Versican (VS) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Versican (Vcan)

Autre désignation: Versican (Vcan Produits)

Synonymes: br146 Kit CLIA, cspg2 Kit CLIA, cspg2a Kit CLIA, sb:cb63 Kit CLIA, versican Kit CLIA, CSPG2 Kit CLIA, Cspg2 Kit CLIA, wgn Kit CLIA, ervr Kit CLIA, pg-m Kit CLIA, wgn1 Kit CLIA, Xversican Kit CLIA, dcan Kit CLIA, cspg2b Kit CLIA, fb99b07 Kit CLIA, wu:fb99b07 Kit CLIA, ERVR Kit CLIA, GHAP Kit CLIA, PG-M Kit CLIA, WGN Kit CLIA, WGN1 Kit CLIA, 5430420N07Rik Kit CLIA, 9430051N09 Kit CLIA, DPEAAE Kit CLIA, NG2 Kit CLIA, PG-M(V0) Kit CLIA, PG-M(V1) Kit CLIA, hdf Kit CLIA, versican a Kit CLIA, versican Kit CLIA, versican b Kit CLIA, vcana Kit CLIA, VCAN Kit CLIA, Vcan Kit CLIA, vcan Kit CLIA, vcanb Kit CLIA

Sujet:

Gene Name: Versican

Gene Aliases: CSPG2, VCAN, ERVR, PG-M, WGN1, Chondroitin Sulfate Proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding, Large fibroblast proteoglycan

ID gène: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process