WNT11 Kit ELISA

N° du produit ABIN5660002

Détail du produit WNT11 Kit ELISA

Antigène: WNT11 (Wingless-Type MMTV Integration Site Family, Member 11 (WNT11))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 11 (WNT11). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 11 (WNT11) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 11 (WNT11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 11 (WNT11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 11 (WNT11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 11 (WNT11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 11 (WNT11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 11 (WNT11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WNT11 (Wingless-Type MMTV Integration Site Family, Member 11 (WNT11))

Autre désignation: Wingless Type MMTV Integration Site Family, Member 11 (WNT11 Produits)

Synonymes: HWNT11 Kit ELISA, Wnt11r Kit ELISA, Xwnt11 Kit ELISA, Xwnt-11 Kit ELISA, wnt11-r Kit ELISA, XWnt-11 Kit ELISA, wnt11b Kit ELISA, wnt11b-2 Kit ELISA, Slb/Wnt11 Kit ELISA, cb748 Kit ELISA, slb Kit ELISA, Wnt12 Kit ELISA, WNT11 Kit ELISA, wnt-11 Kit ELISA, xwnt11 Kit ELISA, Wnt family member 11 Kit ELISA, wingless-type MMTV integration site family member 11 L homeolog Kit ELISA, wingless-type MMTV integration site family, member 11B Kit ELISA, wingless-type MMTV integration site family, member 11 Kit ELISA, protein Wnt-11-like Kit ELISA, wingless-type MMTV integration site family, member 10B Kit ELISA, wingless-type MMTV integration site family, member 11B L homeolog Kit ELISA, WNT11 Kit ELISA, Wnt11 Kit ELISA, wnt11.L Kit ELISA, wnt11b Kit ELISA, wnt11 Kit ELISA, LOC100229189 Kit ELISA, Wnt10b Kit ELISA, wnt11b.L Kit ELISA

Sujet:

Gene Name: Wingless Type MMTV Integration Site Family, Member 11

ID gène: 7481

UniProt: O96014

Pathways: Signalisation WNT, Cell-Cell Junction Organization