ATP2B2 Kit ELISA

N° du produit ABIN5674663

Détail du produit ATP2B2 Kit ELISA

Antigène: ATP2B2 (ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0112 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ATP2B2 (ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2))

Abstract: ATP2B2 Produits

Synonymes: PMCA2 Kit ELISA, PMCA2a Kit ELISA, PMCA2i Kit ELISA, ATP2B4 Kit ELISA, ATP2B2 Kit ELISA, PMCA Kit ELISA, D6Abb2e Kit ELISA, Tmy Kit ELISA, dfw Kit ELISA, jog Kit ELISA, wms Kit ELISA, wri Kit ELISA, ATPase plasma membrane Ca2+ transporting 2 Kit ELISA, ATPase, Ca++ transporting, plasma membrane 2 Kit ELISA, ATP2B2 Kit ELISA, Atp2b2 Kit ELISA, atp2b2 Kit ELISA

Sujet: Alternative name: PMCA2, Plasma Membrane Ca2+ Pump 2, Plasma Membrane Calcium-Transporting ATPase 2

ID gène: 491

UniProt: Q01814

Pathways: Sensory Perception of Sound, Regulation of Cell Size, Ribonucleoside Biosynthetic Process