ATP6AP2 Kit ELISA

N° du produit ABIN5674752

Détail du produit ATP6AP2 Kit ELISA

Antigène: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0055 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Abstract: ATP6AP2 Produits

Synonymes: APT6M8-9 Kit ELISA, ATP6IP2 Kit ELISA, ATP6M8-9 Kit ELISA, ELDF10 Kit ELISA, M8-9 Kit ELISA, MRXE Kit ELISA, MSTP009 Kit ELISA, XMRE Kit ELISA, (P)RR Kit ELISA, 5730403E06Rik Kit ELISA, Atp6ip2 Kit ELISA, tj6 Kit ELISA, wss Kit ELISA, arcl Kit ELISA, j6b7 Kit ELISA, stv1 Kit ELISA, tj6m Kit ELISA, tj6s Kit ELISA, vph1 Kit ELISA, atp6a2 Kit ELISA, atp6n1d Kit ELISA, ATP6AP2 Kit ELISA, DKFZp459G0918 Kit ELISA, atp6ap2 Kit ELISA, wu:fj36c03 Kit ELISA, wu:fq15e04 Kit ELISA, zgc:73194 Kit ELISA, ATPase H+ transporting accessory protein 2 Kit ELISA, ATPase, H+ transporting, lysosomal accessory protein 2 Kit ELISA, ATPase H+ transporting V0 subunit d2 Kit ELISA, ATPase H+ transporting V0 subunit a2 Kit ELISA, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog Kit ELISA, ATP6AP2 Kit ELISA, Atp6ap2 Kit ELISA, ATP6V0D2 Kit ELISA, atp6v0a2 Kit ELISA, atp6ap2.S Kit ELISA, atp6ap2 Kit ELISA

Sujet: Alternative name: ATP6IP2, CAPER, ELDF10, XMRE, Renin/prorenin receptor, Embryonic liver differentiation factor 10, Vacuolar ATP synthase membrane sector-associated protein M8-9

ID gène: 70495

UniProt: Q9CYN9

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones