TRPM4 Kit ELISA

N° du produit ABIN5674771

Détail du produit TRPM4 Kit ELISA

Antigène: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0055 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Autre désignation: Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) (TRPM4 Produits)

Synonymes: PFHB1B Kit ELISA, TRPM4B Kit ELISA, LTrpC-4 Kit ELISA, Mls2s Kit ELISA, 1110030C19Rik Kit ELISA, AW047689 Kit ELISA, LTRPC4 Kit ELISA, TRPM4 Kit ELISA, transient receptor potential cation channel subfamily M member 4 Kit ELISA, transient receptor potential cation channel, subfamily M, member 4 Kit ELISA, TRPM4 Kit ELISA, Trpm4 Kit ELISA

Sujet: Alternative name: TRPM4B, Melastatin-4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4

ID gène: 54795

UniProt: Q8TD43

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response