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CYP11A1 Kit ELISA

CYP11A1 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5674817
  • Antigène Voir toutes CYP11A1 Kits ELISA
    CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))
    Reactivité
    • 2
    • 2
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0133 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes CYP11A1 Kits ELISA
    CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))
    Autre désignation
    Cytochrome P450 11A1 (CYP11A1) (CYP11A1 Produits)
    Synonymes
    CYP11A Kit ELISA, CYP11A1 Kit ELISA, CYPXIA1 Kit ELISA, P450SCC Kit ELISA, Cyp11a Kit ELISA, Cypxia1 Kit ELISA, D9Ertd411e Kit ELISA, P450scc Kit ELISA, Scc Kit ELISA, cscc Kit ELISA, P450(scc) Kit ELISA, SSC Kit ELISA, cytochrome P450 family 11 subfamily A member 1 Kit ELISA, cholesterol side-chain cleavage enzyme, mitochondrial-like Kit ELISA, cytochrome P450 cholesterol side-chain cleavage Kit ELISA, cytochrome P450, family 11, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 11, subfamily A, polypeptide 1 Kit ELISA, CYP11A1 Kit ELISA, LOC101827747 Kit ELISA, Cyp11a1 Kit ELISA
    Sujet
    Alternative name: CYP11A, P450SCC, Cholesterol desmolase, Cholesterol Side Chain Cleavage Enzyme, Cytochrome P450 Family 11 Subfamily A Polypeptide 1
    ID gène
    1583
    UniProt
    P05108
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin
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