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CYP2C19 Kit ELISA

CYP2C19 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5674819
  • Antigène Voir toutes CYP2C19 Kits ELISA
    CYP2C19 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 19 (CYP2C19))
    Reactivité
    • 3
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0056 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes CYP2C19 Kits ELISA
    CYP2C19 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 19 (CYP2C19))
    Autre désignation
    Cytochrome P450 2C19 (CYP2C19) (CYP2C19 Produits)
    Synonymes
    CPCJ Kit ELISA, CYP2C Kit ELISA, P450C2C Kit ELISA, P450IIC19 Kit ELISA, cytochrome P450 family 2 subfamily C member 19 Kit ELISA, cytochrome P450, family 2, subfamily C, polypeptide 19 Kit ELISA, cytochrome P450 2C19 Kit ELISA, CYP2C19 Kit ELISA
    Sujet
    Alternative name: CPCJ, CYP2C, CYPIIC17, CYPIIC19, P450C2C, Cytochrome P450-11A, Cytochrome P450-254C, Mephenytoin 4-hydroxylase, limonene 6-monooxygenase
    ID gène
    1557
    UniProt
    P33261
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