IKBKG Kit ELISA

N° du produit ABIN5674830

Détail du produit IKBKG Kit ELISA

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.67 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Autre désignation: Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma (IkBKg) (IKBKG Produits)

Synonymes: AMCBX1 Kit ELISA, FIP-3 Kit ELISA, FIP3 Kit ELISA, Fip3p Kit ELISA, IKK-gamma Kit ELISA, IP Kit ELISA, IP1 Kit ELISA, IP2 Kit ELISA, IPD2 Kit ELISA, NEMO Kit ELISA, ZC2HC9 Kit ELISA, 1110037D23Rik Kit ELISA, AI848108 Kit ELISA, AI851264 Kit ELISA, AW124339 Kit ELISA, IKK[g] Kit ELISA, Nemo Kit ELISA, nemo Kit ELISA, fj33c07 Kit ELISA, wu:fj33c07 Kit ELISA, zgc:113492 Kit ELISA, MGC97885 Kit ELISA, IKBKG Kit ELISA, IKBKG_1 Kit ELISA, IKBKG_2 Kit ELISA, CG16910 Kit ELISA, DmIKK-gamma Kit ELISA, DmIKKgamma Kit ELISA, Dmel\\CG16910 Kit ELISA, Dmikkgamma Kit ELISA, IKK Kit ELISA, IKK[[gamma]] Kit ELISA, IKKg Kit ELISA, IKKgamma Kit ELISA, KEY Kit ELISA, Kenny Kit ELISA, Key Kit ELISA, dIKK Kit ELISA, dIKK-gamma Kit ELISA, dmIKKgamma Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit gamma Kit ELISA, inhibitor of kappaB kinase gamma Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit gamma L homeolog Kit ELISA, kenny Kit ELISA, IKBKG Kit ELISA, Ikbkg Kit ELISA, ikbkg Kit ELISA, ikbkg.L Kit ELISA, IKBKG_1 Kit ELISA, key Kit ELISA

Sujet: Alternative name: IP, FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, NF-Kappa-B Essential Modulator, Incontinentia Pigmenti, IkB kinase-associated protein 1, NF-kappa-B essential modifier

ID gène: 8517

UniProt: Q9Y6K9

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins