CYP27B1 Kit ELISA

N° du produit ABIN5674843

Détail du produit CYP27B1 Kit ELISA

Antigène: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.29 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Autre désignation: Cytochrome P450 27B1 (CYP27B1) (CYP27B1 Produits)

Synonymes: cp2b Kit ELISA, cyp1 Kit ELISA, cyp1alpha Kit ELISA, cyp27b Kit ELISA, p450c1 Kit ELISA, pddr Kit ELISA, vdd1 Kit ELISA, vddr Kit ELISA, vddri Kit ELISA, vdr Kit ELISA, CP2B Kit ELISA, CYP1 Kit ELISA, CYP1alpha Kit ELISA, CYP27B Kit ELISA, P450c1 Kit ELISA, PDDR Kit ELISA, VDD1 Kit ELISA, VDDR Kit ELISA, VDDRI Kit ELISA, VDR Kit ELISA, Cyp40 Kit ELISA, Cp2b Kit ELISA, Cyp1 Kit ELISA, Cyp27b Kit ELISA, Pddr Kit ELISA, Vdd1 Kit ELISA, Vddr Kit ELISA, VddrI Kit ELISA, Vdr Kit ELISA, cytochrome P450 family 27 subfamily B member 1 Kit ELISA, cytochrome P450, family 27, subfamily B, polypeptide 1 Kit ELISA, cytochrome P450, family 27, subfamily b, polypeptide 1 Kit ELISA, cyp27b1 Kit ELISA, CYP27B1 Kit ELISA, Cyp27b1 Kit ELISA

Sujet: Alternative name: VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase

ID gène: 1594

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D