ATP4b Kit ELISA

N° du produit ABIN5674849

Détail du produit ATP4b Kit ELISA

Antigène: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0136 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Autre désignation: ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) (ATP4b Produits)

Synonymes: AV080843 Kit ELISA, S76401S1 Kit ELISA, ATP6B Kit ELISA, ATPase, H+/K+ exchanging, beta polypeptide Kit ELISA, ATPase H+/K+ transporting beta subunit Kit ELISA, ATPase, H+/K+ exchanging, beta polypeptide S homeolog Kit ELISA, ATPase H+/K+ transporting alpha subunit Kit ELISA, Atp4b Kit ELISA, atp4b.S Kit ELISA, ATP4B Kit ELISA, ATP4A Kit ELISA

Sujet: Alternative name: Proton pump beta chain, Gastric H(+)/K(+) ATPase subunit beta

ID gène: 24217

UniProt: P18598

Pathways: Proton Transport