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ATP1B4 Kit ELISA

ATP1B4 Reactivité: Souris Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Tissue Homogenate
N° du produit ABIN5674854
  • Antigène Voir toutes ATP1B4 Kits ELISA
    ATP1B4 (ATPase, Na+/K+ Transporting, beta 4 Polypeptide (ATP1B4))
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78.125 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78.125 pg/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    27.8 pg/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes ATP1B4 Kits ELISA
    ATP1B4 (ATPase, Na+/K+ Transporting, beta 4 Polypeptide (ATP1B4))
    Autre désignation
    ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) (ATP1B4 Produits)
    Synonymes
    1110020B02Rik Kit ELISA, Ac2-628 Kit ELISA, ATPase, (Na+)/K+ transporting, beta 4 polypeptide Kit ELISA, ATPase Na+/K+ transporting family member beta 4 Kit ELISA, ATPase, Na+/K+ transporting, beta 4 polypeptide L homeolog Kit ELISA, Atp1b4 Kit ELISA, ATP1B4 Kit ELISA, atp1b4.L Kit ELISA
    Sujet
    Alternative name: X,K-ATPase subunit beta-m, X/potassium-transporting ATPase subunit beta-m
    ID gène
    67821
    UniProt
    Q99ME6
    Pathways
    Thyroid Hormone Synthesis, Proton Transport
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