CYP11B2 Kit ELISA

N° du produit ABIN5674859

Détail du produit CYP11B2 Kit ELISA

Antigène: CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0117 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: CYP11B2 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 2 (CYP11B2))

Autre désignation: Aldosterone Synthase (ALDOS) (CYP11B2 Produits)

Synonymes: ALDOS Kit ELISA, CPN2 Kit ELISA, CYP11B Kit ELISA, CYP11BL Kit ELISA, CYPXIB2 Kit ELISA, P-450C18 Kit ELISA, P450C18 Kit ELISA, P450aldo Kit ELISA, Cpn2 Kit ELISA, Cyp11b Kit ELISA, Cyp11b-2 Kit ELISA, Cp45as Kit ELISA, Cyp11b3 Kit ELISA, RNCP45AS Kit ELISA, cytochrome P450 family 11 subfamily B member 2 Kit ELISA, cytochrome P450, family 11, subfamily b, polypeptide 2 Kit ELISA, aldosterone synthase Kit ELISA, cytochrome P450, family 11, subfamily B, polypeptide 2 Kit ELISA, cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2 Kit ELISA, CYP11B2 Kit ELISA, Cyp11b2 Kit ELISA

Sujet: Alternative name: CPN2, CYP11B, CYP11BL, CYP11B2, P450C18, P450aldo, Steroid 18-hydroxylase, Aldosterone-synthesizing enzyme, Cytochrome P450 Family 11 Subfamily B Polypeptide 2

ID gène: 11677

UniProt: P45376

Pathways: ACE Inhibitor Pathway, Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process, Feeding Behaviour