ARPC4 Kit ELISA

N° du produit ABIN5674866

Détail du produit ARPC4 Kit ELISA

Antigène: ARPC4 (Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0115 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ARPC4 (Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4))

Autre désignation: Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) (ARPC4 Produits)

Synonymes: ARC20 Kit ELISA, P20-ARC Kit ELISA, 20kDa Kit ELISA, 5330419I20Rik Kit ELISA, AI327076 Kit ELISA, p20-Arc Kit ELISA, arc20 Kit ELISA, p20-arc Kit ELISA, ARPC4 Kit ELISA, fa07b12 Kit ELISA, wu:fa07b12 Kit ELISA, zgc:77932 Kit ELISA, actin related protein 2/3 complex subunit 4 Kit ELISA, Actin-related protein 2/3 complex subunit 4 Kit ELISA, actin-related protein 2/3 complex subunit 4 Kit ELISA, actin related protein 2/3 complex, subunit 4 Kit ELISA, actin related protein 2/3 complex subunit 4 S homeolog Kit ELISA, actin related protein 2/3 complex, subunit 4, 20kDa Kit ELISA, ARPC4 Kit ELISA, PTRG_00644 Kit ELISA, CMU_000110 Kit ELISA, Arpc4 Kit ELISA, arpc4 Kit ELISA, arpc4.S Kit ELISA

Sujet: Alternative name: ARC20, p20-Arc, Arp2/3 Protein Complex Subunit p20, Arp2/3 complex 20 kDa subunit

ID gène: 100526693

UniProt: P59998

Pathways: Signalisation RTK, Regulation of Actin Filament Polymerization