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ELAVL1 Kit ELISA

ELAVL1 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5674885
  • Antigène Voir toutes ELAVL1 Kits ELISA
    ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
    Reactivité
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0118 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes ELAVL1 Kits ELISA
    ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))
    Autre désignation
    ELAV Like Protein 1 (ELAVL1) (ELAVL1 Produits)
    Synonymes
    ELAV1 Kit ELISA, HUR Kit ELISA, Hua Kit ELISA, MelG Kit ELISA, HuR Kit ELISA, 2410055N02Rik Kit ELISA, W91709 Kit ELISA, elra Kit ELISA, fa25f08 Kit ELISA, fb59a02 Kit ELISA, fc60g02 Kit ELISA, hua Kit ELISA, wu:fa25f08 Kit ELISA, wu:fb59a02 Kit ELISA, wu:fc60g02 Kit ELISA, zHuA Kit ELISA, Elavl1 Kit ELISA, Xel-1 Kit ELISA, elav1 Kit ELISA, elrA Kit ELISA, hur Kit ELISA, melg Kit ELISA, p36 Kit ELISA, ElrA Kit ELISA, elavl1 Kit ELISA, elavl1-a Kit ELISA, elavl1-b Kit ELISA, ELAV like RNA binding protein 1 Kit ELISA, ELAV-like protein 1 Kit ELISA, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) Kit ELISA, ELAV like RNA binding protein 1a Kit ELISA, ELAV like RNA binding protein 1 L homeolog Kit ELISA, ELAV like RNA binding protein 1 S homeolog Kit ELISA, ELAVL1 Kit ELISA, Elavl1 Kit ELISA, elav1 Kit ELISA, elavl1a Kit ELISA, elavl1.L Kit ELISA, elavl1.S Kit ELISA
    Sujet
    Alternative name: ELAV1, HUR, Hua, MelG, Hu Antigen R, Embryonic Lethal,Abnormal Vision Like 1
    ID gène
    1994
    UniProt
    Q15717
    Pathways
    AMPK Signaling
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