PTPN11 Kit ELISA

N° du produit ABIN5674897

Détail du produit PTPN11 Kit ELISA

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.31 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Autre désignation: Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) (PTPN11 Produits)

Synonymes: BPTP3 Kit ELISA, CFC Kit ELISA, NS1 Kit ELISA, PTP-1D Kit ELISA, PTP2C Kit ELISA, SH-PTP2 Kit ELISA, SH-PTP3 Kit ELISA, SHP2 Kit ELISA, 2700084A17Rik Kit ELISA, AW536184 Kit ELISA, PTP1D Kit ELISA, SAP-2 Kit ELISA, SHP-2 Kit ELISA, Shp2 Kit ELISA, Syp Kit ELISA, SYP Kit ELISA, bptp3 Kit ELISA, cfc Kit ELISA, ns1 Kit ELISA, ptp-2 Kit ELISA, ptp2c Kit ELISA, ptpn11 Kit ELISA, ptpn11-a Kit ELISA, ptpn11-b Kit ELISA, shp-2 Kit ELISA, shp2 Kit ELISA, fa14b09 Kit ELISA, wu:fa14b09 Kit ELISA, wu:fi24f03 Kit ELISA, zgc:55388 Kit ELISA, zgc:63553 Kit ELISA, protein tyrosine phosphatase, non-receptor type 11 Kit ELISA, protein tyrosine phosphatase, non-receptor type 11 S homeolog Kit ELISA, protein tyrosine phosphatase, non-receptor type 11, a Kit ELISA, protein tyrosine phosphatase, non-receptor type 11, b Kit ELISA, PTPN11 Kit ELISA, Ptpn11 Kit ELISA, ptpn11.S Kit ELISA, ptpn11a Kit ELISA, ptpn11b Kit ELISA

Classe de substances: Viral Protein

Sujet: Alternative name: BPTP3, CFC, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, Noonan Syndrome 1, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C

ID gène: 19247

UniProt: P35235

Pathways: Signalistation JAK/STAT, Signalisation RTK, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, L'effet Warburg