SLC9A3 Kit ELISA

N° du produit ABIN5674915

Détail du produit SLC9A3 Kit ELISA

Antigène: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0093 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Autre désignation: Sodium/Hydrogen Exchanger 3 (NHE3) (SLC9A3 Produits)

Synonymes: SLC9A3 Kit ELISA, 9030624O13Rik Kit ELISA, AI930210 Kit ELISA, NHE-3 Kit ELISA, NHE3 Kit ELISA, Nhe3 Kit ELISA, solute carrier family 9 member A3 Kit ELISA, sodium/hydrogen exchanger 3 Kit ELISA, Sodium/hydrogen exchanger 3 Kit ELISA, putative sodium/hydrogen exchanger 3 (nhe3) Kit ELISA, solute carrier family 9 (sodium/hydrogen exchanger), member 3 Kit ELISA, SLC9A3 Kit ELISA, LOC5570922 Kit ELISA, GL50803_102647 Kit ELISA, Smp_045680 Kit ELISA, Slc9a3 Kit ELISA

Sujet: Alternative name: SLC9A3, Solute Carrier Family 9 Member 3, Sodium-hydrogen antiporter 3, Na(+)/H(+) exchanger 3

ID gène: 6550

UniProt: P48764

Pathways: Proton Transport