PPP2R4 Kit ELISA

N° du produit ABIN5674923

Détail du produit PPP2R4 Kit ELISA

Antigène: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Épitope: Regulatory Subunit 4

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0062 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Autre désignation: Protein Phosphatase 2A Activator, (PPP2R4) (PPP2R4 Produits)

Synonymes: PP2A Kit ELISA, PR53 Kit ELISA, PTPA Kit ELISA, 2610042B21Rik Kit ELISA, C77440 Kit ELISA, N28142 Kit ELISA, PTPRA Kit ELISA, Ppp2r4 Kit ELISA, fc23d07 Kit ELISA, ppp2r4 Kit ELISA, wu:fc23d07 Kit ELISA, zgc:92766 Kit ELISA, pp2a Kit ELISA, ppp2r4.L Kit ELISA, pr53 Kit ELISA, ptpa Kit ELISA, protein phosphatase 2 phosphatase activator Kit ELISA, protein phosphatase 2 protein activator Kit ELISA, protein phosphatase 2 phosphatase activator L homeolog Kit ELISA, PTPA Kit ELISA, Ptpa Kit ELISA, ptpa Kit ELISA, ptpa.L Kit ELISA

Sujet: Alternative name: PP2A, PR53, PTPA, Phosphotyrosyl Phosphatase Activator, PP2A Phosphatase Activator, PP2A, subunit B', PR53 isoform, Serine/threonine-protein phosphatase 2A activator

ID gène: 110854

UniProt: P58389

Pathways: Signalisation PI3K-Akt, M Phase, Hepatitis C, Toll-Like Receptors Cascades