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JKAMP Kit ELISA

JKAMP Reactivité: Souris Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Tissue Homogenate
N° du produit ABIN5674945
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    JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
    Reactivité
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.781 ng/mL - 50 ng/mL
    Seuil minimal de détection
    0.781 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0.26 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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    Discover our top product JKAMP Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes JKAMP Kits ELISA
    JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
    Autre désignation
    JNK1/MAPK8 Associated Membrane Protein (JKAMP) (JKAMP Produits)
    Synonymes
    C14orf100 Kit ELISA, HSPC213 Kit ELISA, HSPC327 Kit ELISA, JAMP Kit ELISA, 1200003C05Rik Kit ELISA, 2310047L11Rik Kit ELISA, Jamp Kit ELISA, RGD1308917 Kit ELISA, JNK1/MAPK8 associated membrane protein Kit ELISA, JNK1/MAPK8-associated membrane protein Kit ELISA, JKAMP Kit ELISA, Jkamp Kit ELISA
    Sujet
    Alternative name: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4
    ID gène
    104771
    UniProt
    Q8BI36
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