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SSB Kit ELISA

SSB Reactivité: Rat Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5674951
  • Antigène Voir toutes SSB Kits ELISA
    SSB (Sjogren Syndrome Antigen B (SSB))
    Reactivité
    • 3
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78.12 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78.12 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    29 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes SSB Kits ELISA
    SSB (Sjogren Syndrome Antigen B (SSB))
    Abstract
    SSB Produits
    Synonymes
    LARP3 Kit ELISA, La Kit ELISA, lab1 Kit ELISA, larp3 Kit ELISA, ssb Kit ELISA, Mt-SSB Kit ELISA, MtSSB Kit ELISA, Ssbp1 Kit ELISA, SS-B Kit ELISA, laa-a Kit ELISA, ssb-a Kit ELISA, ssb-b Kit ELISA, wu:fb17f11 Kit ELISA, zgc:55588 Kit ELISA, Sjogren syndrome antigen B Kit ELISA, Sjogren syndrome antigen B L homeolog Kit ELISA, single stranded DNA binding protein 1 Kit ELISA, Sjogren syndrome antigen B S homeolog Kit ELISA, Sjogren syndrome antigen B (autoantigen La) Kit ELISA, SSB Kit ELISA, ssb.L Kit ELISA, SSBP1 Kit ELISA, Ssb Kit ELISA, ssb.S Kit ELISA, ssb Kit ELISA
    Sujet
    Alternative name: LARP3, Lupus La Protein, Autoantigen La, La Ribonucleoprotein Domain Family,Member 3, Autoantigen SSB/La
    UniProt
    P38656
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