HIF1A Kit ELISA

N° du produit ABIN5690734

Détail du produit HIF1A Kit ELISA

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Human Hydroxylated-HIF-1 alpha (P402) Cell-based ELISA Kit. This ELISA is for measuring hydroxylated HIF-1 alpha (Pro-402) in human adherent cell lines.

Type d'échantillon: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human Hydroxylated-HIF-1alpha hydroxylated at site Proline-402

Attributs du produit:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Ingrédients:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Plaque: Uncoated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Autre désignation: HIF-1alpha (HIF1A Produits)

Synonymes: hif1a Kit ELISA, AA959795 Kit ELISA, HIF1alpha Kit ELISA, MOP1 Kit ELISA, bHLHe78 Kit ELISA, HIF-1A Kit ELISA, HIF-1alpha Kit ELISA, HIF1 Kit ELISA, HIF1-ALPHA Kit ELISA, PASD8 Kit ELISA, HIF1-alpha Kit ELISA, hif-1a Kit ELISA, hypoxia inducible factor 1 alpha Kit ELISA, hypoxia inducible factor 1, alpha subunit Kit ELISA, hypoxia inducible factor 1 alpha subunit Kit ELISA, HIF (Hypoxia Inducible Factor) homologa Kit ELISA, hif-1a Kit ELISA, Hif1a Kit ELISA, HIF1A Kit ELISA, hif1a Kit ELISA, hif-1 Kit ELISA

ID gène: 3091

UniProt: Q16665

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg