CGRP Kit ELISA

N° du produit ABIN5690747

Détail du produit CGRP Kit ELISA

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human/Mouse/Rat CGRP-I EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Dynorphin

Sensibilité: 0.2 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Autre désignation: CGRP-I (CALCA Produits)

Synonymes: CALC1 Kit ELISA, CGRP Kit ELISA, CGRP-I Kit ELISA, CGRP1 Kit ELISA, CT Kit ELISA, KC Kit ELISA, CALC Kit ELISA, CALCA Kit ELISA, CALC3 Kit ELISA, ci-ct Kit ELISA, calca Kit ELISA, CA Kit ELISA, CGRP-1 Kit ELISA, Calc Kit ELISA, Calc1 Kit ELISA, Cgrp Kit ELISA, Ct Kit ELISA, Ctn Kit ELISA, calcitonin Kit ELISA, CAL6 Kit ELISA, Cal1 Kit ELISA, RATCAL6 Kit ELISA, CGRPI Kit ELISA, calc Kit ELISA, cgrp Kit ELISA, zgc:92886 Kit ELISA, calcitonin related polypeptide alpha Kit ELISA, calcitonin related polypeptide beta Kit ELISA, calcitonin-related polypeptide alpha Kit ELISA, calcitonin Kit ELISA, calcitonin/calcitonin-related polypeptide, alpha Kit ELISA, calcitonin-related polypeptide beta Kit ELISA, calcitonin gene-related peptide 2 Kit ELISA, CALCA Kit ELISA, CALCB Kit ELISA, ci-ct Kit ELISA, calca Kit ELISA, Calca Kit ELISA, LOC100008771 Kit ELISA

ID gène: 24241

UniProt: P01256

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour