IKBKG Kit ELISA
IKBKG
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690893
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Antigène
Voir toutes IKBKG Kits ELISA
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IKBKG
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human IKK-gamma/NEMO/FIP-3 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificité
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This ELISA antibody pair recognizes Human IKK-gamma.
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Stock
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4 °C
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Date de péremption
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6 months
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Antigène
Voir toutes IKBKG Kits ELISA
-
IKBKG
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))
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Autre désignation
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IKK-gamma (NEMO/FIP-3) (IKBKG Produits)
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Synonymes
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AMCBX1 Kit ELISA, FIP-3 Kit ELISA, FIP3 Kit ELISA, Fip3p Kit ELISA, IKK-gamma Kit ELISA, IP Kit ELISA, IP1 Kit ELISA, IP2 Kit ELISA, IPD2 Kit ELISA, NEMO Kit ELISA, ZC2HC9 Kit ELISA, 1110037D23Rik Kit ELISA, AI848108 Kit ELISA, AI851264 Kit ELISA, AW124339 Kit ELISA, IKK[g] Kit ELISA, Nemo Kit ELISA, nemo Kit ELISA, fj33c07 Kit ELISA, wu:fj33c07 Kit ELISA, zgc:113492 Kit ELISA, MGC97885 Kit ELISA, IKBKG Kit ELISA, IKBKG_1 Kit ELISA, IKBKG_2 Kit ELISA, CG16910 Kit ELISA, DmIKK-gamma Kit ELISA, DmIKKgamma Kit ELISA, Dmel\\CG16910 Kit ELISA, Dmikkgamma Kit ELISA, IKK Kit ELISA, IKK[[gamma]] Kit ELISA, IKKg Kit ELISA, IKKgamma Kit ELISA, KEY Kit ELISA, Kenny Kit ELISA, Key Kit ELISA, dIKK Kit ELISA, dIKK-gamma Kit ELISA, dmIKKgamma Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit gamma Kit ELISA, inhibitor of kappaB kinase gamma Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit gamma L homeolog Kit ELISA, kenny Kit ELISA, IKBKG Kit ELISA, Ikbkg Kit ELISA, ikbkg Kit ELISA, ikbkg.L Kit ELISA, IKBKG_1 Kit ELISA, key Kit ELISA
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ID gène
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8517
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UniProt
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Q9Y6K9
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Pathways
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Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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