Reactive Oxygen Species (ROS) Fluorometric Assay Kit

N° du produit ABIN5708944

Aperçu rapide pour Reactive Oxygen Species (ROS) Fluorometric Assay Kit (ABIN5708944)

Antigène: Reactive Oxygen Species (ROS)

Méthode de détection: Fluorometric

Application: Biochemical Assay (BCA)

Type d'échantillon: Cell Culture Cells, Tissue Samples

Analytical Method: Quantitative

Détail du produit

Fonction: This kit can be used for detection of ROS content in cells and tissue samples.

Attributs du produit: DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

Matériel non inclus: Fluorescence Microplate reader, Fluorescence Spectrophotometry, Flow Cytometry

Information d'application

Durée du test: 1 h

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect from light.

Stock: 4 °C,-20 °C

Stockage commentaire: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Date de péremption: 12 months

Détail du antigène

Antigène: Reactive Oxygen Species (ROS)

Autre désignation: Reactive Oxygen Species