TNFRSF1A Kit ELISA

N° du produit ABIN625089

Détail du produit TNFRSF1A Kit ELISA

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1-400 pg/mL

Seuil minimal de détection: 1 pg/mL

Application: ELISA

Fonction: Human TNF RI (TNFRSF1A) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensibilité: 1 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples20 - 200 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour TNFRSF1A Kit ELISA (ABIN625089)

Fernández-Juárez, Villacorta Perez, Luño Fernández, Martinez-Martinez, Cachofeiro, Barrio Lucia, Tato Ribera, Mendez Abreu, Cordon, Oliva Dominguez, Praga Terente: "High levels of circulating TNFR1 increase the risk of all-cause mortality and progression of renal disease in type 2 diabetic nephropathy." dans: Nephrology (Carlton, Vic.), Vol. 22, Issue 5, pp. 354-360, (2017) (PubMed).

Gao, Camous, Lu, Lim, Larbi, Ng: "Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies." dans: Neurobiology of aging, Vol. 39, pp. 140-6, (2016) (PubMed).

Wu, Ding, Han, Arriens, Wei, Han, Pedroza, Jiang, Anolik, Petri, Sanz, Saxena, Mohan: "Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study." dans: Journal of proteome research, Vol. 15, Issue 7, pp. 2102-14, (2016) (PubMed).

Sennikov, Alshevskaya, Shkaruba, Chumasova, Sizikov, Lopatnikova: "Expression of TNF? membrane-bound receptors in the peripheral blood mononuclear cells (PMBC) in rheumatoid arthritis patients." dans: Cytokine, Vol. 73, Issue 2, pp. 288-94, (2015) (PubMed).

Bishayi, Bandyopadhyay, Majhi, Adhikary et al.: "Expression of CXCR1 (interleukin-8 receptor) in murine macrophages after staphylococcus aureus infection and its possible implication on intracellular survival correlating with cytokines and ..." dans: Inflammation, Vol. 38, Issue 2, pp. 812-27, (2015) (PubMed).

Golikova, Lopatnikova, Kovalevskaya-Kucheryavenko, Nepomnyashih, Sennikov: "Levels of TNF, TNF autoantibodies and soluble TNF receptors in patients with bronchial asthma." dans: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 50, Issue 7, pp. 705-11, (2014) (PubMed).

Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." dans: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).

Issersohn: "[Radiologic examination of the colon with double contrast medium according to the Welin method]." dans: Dapim refuiim. Folia medica, Vol. 24, Issue 1, pp. 27-31 (PubMed).

Détail du antigène

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: TNF RI (TNFRSF1A Produits)

Synonymes: CD120a Kit ELISA, FPF Kit ELISA, MS5 Kit ELISA, TBP1 Kit ELISA, TNF-R Kit ELISA, TNF-R-I Kit ELISA, TNF-R55 Kit ELISA, TNFAR Kit ELISA, TNFR1 Kit ELISA, TNFR1-d2 Kit ELISA, TNFR55 Kit ELISA, TNFR60 Kit ELISA, p55 Kit ELISA, p55-R Kit ELISA, p60 Kit ELISA, TNF-R1 Kit ELISA, TNF-alphaR1 Kit ELISA, TNFRI Kit ELISA, TNFRp55 Kit ELISA, TNFalpha-R1 Kit ELISA, Tnfr-2 Kit ELISA, Tnfr1 Kit ELISA, fpf Kit ELISA, tbp1 Kit ELISA, p55-r Kit ELISA, tnf-r Kit ELISA, tnfar Kit ELISA, tnfr1 Kit ELISA, cd120a Kit ELISA, tnfr55 Kit ELISA, tnfr60 Kit ELISA, xtnfr1 Kit ELISA, wu:fj65b10 Kit ELISA, zgc:85914 Kit ELISA, TNFR I Kit ELISA, TNFRSF1A Kit ELISA, TNFA Kit ELISA, cTNF Kit ELISA, TNF receptor superfamily member 1A Kit ELISA, tumor necrosis factor receptor superfamily, member 1a Kit ELISA, TNF receptor superfamily member 1A S homeolog Kit ELISA, tumor necrosis factor Kit ELISA, TNFRSF1A Kit ELISA, Tnfrsf1a Kit ELISA, tnfrsf1a.S Kit ELISA, tnfrsf1a Kit ELISA, TNF Kit ELISA

Sujet: Gene Names: TNFRSF1A TNFAR TNFR1
Protein names: Tumor necrosis factor receptor superfamily member 1A (Tumor necrosis factor receptor 1) (TNF-R1) (Tumor necrosis factor receptor type I) (TNF-RI) (TNFR-I) (p55) (p60) (CD antigen CD120a) [Cleaved into: Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor-binding protein 1 (TBPI)]

ID gène: 7132

UniProt: P19438

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway