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CXCL16 Kit ELISA

CXCL16 Reactivité: Souris Colorimetric Sandwich ELISA 1.3-80 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625119
  • Antigène Voir toutes CXCL16 Kits ELISA
    CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
    Reactivité
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.3-80 pg/mL
    Seuil minimal de détection
    1.3 pg/mL
    Application
    ELISA
    Fonction
    Mouse CXCL16 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Serum, Plasma, Cell Culture Supernatant
    Analytical Method
    Quantitative
    Specificité
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    Sensibilité
    < 1.3 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling CXCL16 Kit ELISA
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    Discover our top product CXCL16 Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples10 - 100 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation des réactifs
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 10-100 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
      4. Preparation of standard: Briefly spin the vial of Item C and then add 800 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare a 40 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 4 µL CXCL16 standard from the vial of Item C (40 ng/mL), into a tube with 1996 µL Assay Diluent A or 1x Assay Diluent B to prepare a 80 pg/mL stock standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 300 µL 300myl 4 µL standard + 1996 µL 300 µL 300 µL 300 µL 300 µL 80 40 20 10 5 2.5 1.25 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
    Procédure de l'essai
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    Calcul des résultats

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse CXCL16 concentration (pg/mL) 1 10 100 O D =4 50 n m 0.1 1 10 Assay Diluent B Mouse CXCL16 concentration (pg/mL) 1 10 100 O D =4 50 n m 0.1 1 10
    Sensitivity: The minimum detectable dose of CXCL16 is typically less than 1.3 pg/mL.
    Recovery: Recovery was determined by spiking various levels of mouse CXCL16 into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 94.34 83-103 Plasma 93.49 82-102 Cell culture media 96.71 84-103
    Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 93 94 94 Range ( %) 83-103 82-103 83-103 1:4 Average % of Expected 94 93 96 Range ( %) 84-103 83-102 84-103
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    Précision du teste
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Vergori, Lauret, Gaceb, Beauvillain, Andriantsitohaina, Martinez: "PPAR? regulates endothelial progenitor cell maturation and myeloid lineage differentiation through a NADPH oxidase-dependent mechanism in mice." dans: Stem cells (Dayton, Ohio), Vol. 33, Issue 4, pp. 1292-303, (2015) (PubMed).

    Han, Lee, Ryu, Choi: "Tumor-conditioned Gr-1(+)CD11b(+) myeloid cells induce angiogenesis through the synergistic action of CCL2 and CXCL16 in vitro." dans: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1218-25, (2014) (PubMed).

    Zeng, See, Phallen, Jackson, Belcaid, Ruzevick, Durham, Meyer, Harris, Albesiano, Pradilla, Ford, Wong, Hammers, Mathios, Tyler, Brem, Tran, Pardoll, Drake, Lim: "Anti-PD-1 blockade and stereotactic radiation produce long-term survival in mice with intracranial gliomas." dans: International journal of radiation oncology, biology, physics, Vol. 86, Issue 2, pp. 343-9, (2013) (PubMed).

  • Antigène Voir toutes CXCL16 Kits ELISA
    CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))
    Autre désignation
    CXCL16 (CXCL16 Produits)
    Synonymes
    CXCL16 Kit ELISA, 0910001K24Rik Kit ELISA, AV290116 Kit ELISA, BB024863 Kit ELISA, CXCL16v1 Kit ELISA, CXCL16v2 Kit ELISA, SR-PSOX Kit ELISA, Zmynd15 Kit ELISA, b2b498Clo Kit ELISA, CXCLG16 Kit ELISA, SRPSOX Kit ELISA, C-X-C motif chemokine ligand 16 Kit ELISA, chemokine (C-X-C motif) ligand 16 Kit ELISA, CXCL16 Kit ELISA, Cxcl16 Kit ELISA
    Sujet
    The Mouse CXCL16 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL16 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse CXCL16 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
    ID gène
    66102
    UniProt
    Q8BSU2
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