Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

DPP4 Kit ELISA

DPP4 Reactivité: Humain Colorimetric Sandwich ELISA 25-8000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625276
  • Antigène Voir toutes DPP4 Kits ELISA
    DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
    Reactivité
    • 9
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    25-8000 pg/mL
    Seuil minimal de détection
    25 pg/mL
    Application
    ELISA
    Fonction
    Human CD26 (DPPIV) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensibilité
    < 25 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling DPP4 Kit ELISA
    Top Product
    Discover our top product DPP4 Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples500 - 5,000 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation des réactifs
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 500-5,000 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator. For example, add 1 µL of serum/plasma into a tube 999 µL Assay Diluent A to prepare a 1,000-fold diluted sample. Mix well.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
      4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates/urine) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 80 µL CD26 standard (50 ng/mL) from the vial of Item C, into a tube with 420 µL Assay Diluent A or 1x Assay Diluent B to prepare a 8,000 pg/mL standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 8,000 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 80 µL standard + 420 µL 200 µL 200 µL 200 µL 200 µL 200 µL 200myl
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of 8,000 3,200 1,280 512 204.8 81.92 32.77 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 500-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 20 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 500-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
    Procédure de l'essai
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    Calcul des résultats

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Human CD26 concentration (pg/mL) 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Human CD26 concentration (pg/mL) 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10
    Sensitivity: The minimum detectable dose of CD26 is typically less than 25 pg/mL.
    Recovery: Recovery was determined by spiking various levels human CD26 into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 122.8 118-134 Plasma 114.4 106-122 Cell culture media 119.5 105-130
    Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 97.20 90.13 94.52 Range ( %) 89-105 77-103 87-101 1:4 Average % of Expected 82.47 84.78 95.87 Range ( %) 74-90 79-89 83-108
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    Précision du teste
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Mukherjee, Mukhopadhyay, Ghosh, Barbhuiya, Das, Chatterjee: "Decreased Frequency and Secretion of CD26 Promotes Disease Progression in Indian Post Kala-azar Dermal Leishmaniasis." dans: Journal of clinical immunology, Vol. 36, Issue 1, pp. 85-94, (2016) (PubMed).

    Ahmed, Huri, Al-Hamodi, Salem, Muniandy: "Serum Levels of Soluble CD26/Dipeptidyl Peptidase-IV in Type 2 Diabetes Mellitus and Its Association with Metabolic Syndrome and Therapy with Antidiabetic Agents in Malaysian Subjects." dans: PLoS ONE, Vol. 10, Issue 10, pp. e0140618, (2015) (PubMed).

    Vos, de Klerk, Krajnc, Kruizinga, van Ommen, Rozing: "Toxicity of bis(tri-n-butyltin)oxide in the rat. II. Suppression of thymus-dependent immune responses and of parameters of nonspecific resistance after short-term exposure." dans: Toxicology and applied pharmacology, Vol. 75, Issue 3, pp. 387-408, (1984) (PubMed).

  • Antigène Voir toutes DPP4 Kits ELISA
    DPP4 (Dipeptidyl-Peptidase 4 (DPP4))
    Autre désignation
    DPPIV / CD26 (DPP4 Produits)
    Synonymes
    ADABP Kit ELISA, ADCP2 Kit ELISA, CD26 Kit ELISA, DPPIV Kit ELISA, TP103 Kit ELISA, MGC81966 Kit ELISA, MOP9.8 Kit ELISA, MOP9_8 Kit ELISA, DPP4 Kit ELISA, si:ch73-2d23.3 Kit ELISA, Cd26 Kit ELISA, Dpp-4 Kit ELISA, THAM Kit ELISA, dipeptidyl peptidase 4 Kit ELISA, dipeptidyl-peptidase 4 S homeolog Kit ELISA, prolyl oligopeptidase family protein Kit ELISA, dipeptidyl-peptidase 4 Kit ELISA, dipeptidylpeptidase 4 Kit ELISA, DPP4 Kit ELISA, dpp4.S Kit ELISA, AT5G24260 Kit ELISA, dpp4 Kit ELISA, Dpp4 Kit ELISA
    ID gène
    1803
    UniProt
    P27487
    Pathways
    Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
Vous êtes ici:
Support technique