Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

MME Kit ELISA

MME Reactivité: Souris Colorimetric Sandwich ELISA 61-15000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625421
  • Antigène Voir toutes MME Kits ELISA
    MME (Membrane Metallo-Endopeptidase (MME))
    Reactivité
    • 4
    • 4
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    61-15000 pg/mL
    Seuil minimal de détection
    61 pg/mL
    Application
    ELISA
    Fonction
    Mouse Neprilysin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes mouse Neprilysin.
    Sensibilité
    61 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling MME Kit ELISA
    Top Product
    Discover our top product MME Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes MME Kits ELISA
    MME (Membrane Metallo-Endopeptidase (MME))
    Autre désignation
    Neprilysin (MME Produits)
    Synonymes
    CALLA Kit ELISA, CD10 Kit ELISA, NEP Kit ELISA, SFE Kit ELISA, 6030454K05Rik Kit ELISA, C85356 Kit ELISA, Nep Kit ELISA, neprilysin Kit ELISA, membrane metalloendopeptidase Kit ELISA, membrane metallo endopeptidase Kit ELISA, membrane metallo-endopeptidase Kit ELISA, MME Kit ELISA, Mme Kit ELISA
    Classe de substances
    Chemical
    Sujet
    Gene Names: Mme
    Protein names: Neprilysin (EC 3.4.24.11) (Atriopeptidase) (Enkephalinase) (Neutral endopeptidase 24.11) (NEP) (Neutral endopeptidase) (Skin fibroblast elastase) (SFE) (CD antigen CD10)
    ID gène
    17380
    UniProt
    Q61391
    Pathways
    Signalisation RTK, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
Vous êtes ici:
Support technique