RBP4 Kit IQ-ELISA (Retinol Binding Protein 4, Plasma)

Details for Product RBP4 Kit IQ-ELISA No. ABIN6384915
Antigène
  • RDCCAS
  • rbp4
  • MGC54038
  • srbp
  • RBP
  • PRBP
  • RBPA
  • Rbp-4
  • fb23c12
  • rbp
  • wu:fb23c12
  • wu:fb58d04
  • wu:fb72b04
  • zgc:86911
  • retinol binding protein 4
  • retinol binding protein 4 S homeolog
  • retinol binding protein 4 A, plasma
  • retinol binding protein 4, plasma
  • retinol binding protein 4 L homeolog
  • RBP4
  • rbp4.S
  • rbp4
  • Rbp4
  • RBP4A
  • rbp4.L
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
8
7
6
6
5
2
2
2
2
2
1
1
1
1
Méthode de détection
qPCR
Type de méthode
Sandwich ELISA
Application
ELISA
Options
Fonction Mouse Immunoquantitative (PCR-Based) RBP4 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon Cell Culture Supernatant, Plasma, Serum
Analytical Method Semi-Quantitative
Méthode de détection qPCR
Attributs du produit IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
Ingrédients
  • RBP4 Microplate (Item A): 96 well PCR plate coated with anti-Mouse RBP4
  • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
  • Standards (Item C): 2 vials of recombinant Mouse RBP4
  • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
  • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
  • Detection Affinity Reagent for RBP4 (Item F): 2 vials of a 4x concentrated solution of anti-Mouse RBP4 affinity reagent
  • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
  • Primer Solution (Item I): 1.7 ml vial
  • PCR Master Mix (Item J): 1.2 ml vial
  • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
  • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
Matériel non inclus
  • Real-time PCR instrument, Bio-Rad recommended
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Absorbent paper
  • Distilled or deionized water
  • Log-log graph paper or computer and software for data analysis
  • Tubes to prepare standard or sample dilutions
  • Heating block or water bath capable of 80°C
Plasmids, Primers & others Plasmids, Primers & others RBP4 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation RBP4 (RBP4 ELISA Kit Extrait)
ID gène 19662
UniProt Q00724
Pathways Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
Indications d'application The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
Commentaires

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon 25 µL
Plaque Pre-coated
Protocole
  1. Prepare all reagents, samples and standards as instructed
  2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
  3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
  4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
  5. Add 15μL Primer solution 10μL of PCR master mix to each well
  6. Run real-time PCR
Restrictions For Research Use only
Stock 4 °C,-20 °C,-80 °C
Stockage commentaire May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Date de péremption 6 months
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