CST Kit ELISA (Cortistatin)

Details for Product CORT Kit ELISA No. ABIN6574088
Toutes les espèces
Kits with alternative reactivity to:
Méthode de détection
Type de méthode
Competition ELISA
Gamme de detection
1.23 ng/mL - 100 ng/mL
Seuil minimal de détection
1.23 ng/mL
Fonction The Cortistatin / CORT ELISA kit is an enzyme immunoassay for the in vitro detection of CORT in serum, plasma, Urine, biological fluids

We offer validation data (WB) for each of the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
Type d'échantillon Plasma, Serum, Urine
Analytical Method Quantitative
Méthode de détection Colorimetric

This assay has high sensitivity and excellent specificity for detection of Corticosterone (Cort).
No significant cross-reactivity or interference between Corticosterone (Cort) and analogues was observed.

Réactivité croisée (Details) No significant cross-reactivity or interference between Corticosterone (Cort) and analogues was observed.
Sensibilité 0.51 ng/mL
  • Pre-coated, ready to use 96-well strip plate
  • Plate sealer for 96 wells
  • Standard Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Stop Solution
  • Standard
  • Detection Reagent A
  • Detection Reagent B
  • TMB Substrate
  • Wash Buffer (30 x concentrate)
  • Instruction manual
Matériel non inclus
  • Microplate reader with 450 nm filter.
  • Precision single or multi-channel pipettes and disposable tips.
  • Eppendorf Tubes for diluting samples.
  • Deionized or distilled water.
  • Absorbent paper for blotting the microtiter plate.
  • Container for Wash Solution
Autre désignation CORT (CORT ELISA Kit Extrait)
Indications d'application
  • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
  • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
  • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
  • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
  • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
  • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
  • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
  • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
  • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
  • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon 50 μL
Durée du test 2 h
Plaque Pre-coated
Protocole This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticosterone (Cort) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corticosterone (Cort) and unlabeled Corticosterone (Cort) (Standards or samples) with the pre-coated antibody specific to Corticosterone (Cort). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticosterone (Cort) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticosterone (Cort) in the sample.
Préparation des réactifs
  • Bring all kit components and samples to room temperature (18-25 °C) before use.
  • Standard - Reconstitute the Standard with 0.3 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 500 ng/mL. Please prepare 5 tubes containing 0.2 mL Standard Diluent and produce a triple dilution series. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 500 ng/mL, 166.7 ng/mL, 55.6 ng/mL, 18.5 ng/mL, 6.17 ng/mL, and the last EP tubes with Standard Diluent is the blank as 0 ng/mL.
  • Assay Diluent A and Assay Diluent B - Dilute 6 mL of Assay Diluent A or B Concentrate(2x) with 6 mL of deionized or distilled water to prepare 12 mL of Assay Diluent A or B. (In fact, more than 6 mL Assay Diluent A and Assay Diluent B are contained in the bottles. Therefore, in every test, please precisely pipette required amount of Diluent and make double dilution in a new container. The prepared working dilution cannot be frozen.)
  • Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with working Assay Diluent A or B, respectively (1:100).
  • Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
  • TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.
  • Making serial dilution in the wells directly is not permitted.
  • Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
  • Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
  • Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
  • The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
  • If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
  • Contaminated water or container for reagent preparation will influence the detection result.
Prélèvement de l'échantillon Serum: Allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 20 minutes at approximately 1000 x g. Assay immediately or store samples in aliquot at -20 °C or -80 °C. Avoid repeated freeze/thaw cycles.

Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20 °C or -80 °C. Avoid repeated freeze/thaw cycles.

Urine: Aseptically collect the first urine of the day (mid-stream) , voided directly into a sterile container. Centrifuge to remove particulate matter, assay immediately or aliquot and store at -20 °C. Avoid repeated freeze-thaw cycles.

Biological Fluids: Centrifuge samples for 20 minutes at 1000 x g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Préparation de l'échantillon
  • We are only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
  • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. Sample should be diluted by 0.01Mol/L PBS(PH=7.0-7.2).
  • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
  • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals.
  • Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g.antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
  • Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit.
  • Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
Procédure de l'essai
  1. Prepare all reagents, samples and standards,
  2. Add 50μL standard or sample to each well.
    And then add 50μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37 °C,
  3. Aspirate and wash 3 times,
  4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
  5. Aspirate and wash 5 times,
  6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
  7. Add 50μL Stop Solution. Read at 450 nm immediately.
Calcul des résultats

This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between CORT concentration in the sample and the assay signal intensity. Average the duplicate readings for each standard, control, and samples. Create a standard curve on log-log or semi-log graph paper, with the log of CORT concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.

Précision du teste

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticosterone (Cort) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticosterone (Cort) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only
Précaution d'utilisation The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Conseil sur la manipulation The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Stock 4 °C
Stockage commentaire
  • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
  • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
    Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
  • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
Date de péremption 6 months
Image no. 1 for Cortistatin (CORT) ELISA Kit (ABIN6574088) SDS-PAGE of Protein Standard from the Kit (BSA-Cort).
Image no. 2 for Cortistatin (CORT) ELISA Kit (ABIN6574088) Rabbit Capture antibody from the kit in ELISA with Positive Control: Human, rat, mous...
Image no. 3 for Cortistatin (CORT) ELISA Kit (ABIN6574088) Typical standard curve
Produit citée dans: Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." dans: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, 2018 (PubMed).

Lu, Liu, Wang, Sun, Yang, Xing, Sun, Wang, Zhao: "The Role of Psychological Stress on Heart Autophagy in Mice With Heart Failure." dans: Psychosomatic medicine, Vol. 79, Issue 9, pp. 1036-1044, 2018 (PubMed).

Dong, Wang, Qin, Cao, Chen: "Role of serotonin in the intestinal mucosal epithelium barrier in weaning mice undergoing stress-induced diarrhea." dans: Journal of molecular histology, Vol. 49, Issue 1, pp. 85-97, 2018 (PubMed).

Zhao, Zhang, Guo, Cao, Xue, Zhao, Yang, Yang, Ji, Huang, Sun: "Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis." dans: Frontiers in molecular neuroscience, Vol. 10, pp. 293, 2017 (PubMed).

Zhang, Li, Li, Tan, Cheng: "Environmental perturbation of the circadian clock during pregnancy leads to transgenerational mood disorder-like behaviors in mice." dans: Scientific reports, Vol. 7, Issue 1, pp. 12641, 2017 (PubMed).

Zhao, Zhao, Yang, Cao, Zhang, Ji, Gu, Huang, Sun: "Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus." dans: Brain research bulletin, Vol. 130, pp. 146-155, 2017 (PubMed).

Li, Deng, Wang, Fu, You, Tian: "Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed rats." dans: European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, Vol. 26, Issue 3, pp. 420-30, 2016 (PubMed).

Olatunji, Omolekulo, Usman, Kim: "Improvement of oral contraceptive-induced glucose dysregulation and dyslipidemia by valproic acid is independent of circulating corticosterone." dans: Archives of physiology and biochemistry, Vol. 122, Issue 3, pp. 123-9, 2016 (PubMed).

Olatunji, Usman, Seok, Kim: "Activation of cardiac renin-angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder." dans: Archives of physiology and biochemistry, pp. 1-8, 2016 (PubMed).

Abass, Elkhateeb, Abd El-Baset, Kattaia, Mohamed, Atteia: "Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway." dans: Environmental science and pollution research international, Vol. 23, Issue 15, pp. 15262-74, 2016 (PubMed).

Yi, Qi, Li, Le, Shao, Du, Dong: "Moxibustion upregulates hippocampal progranulin expression." dans: Neural regeneration research, Vol. 11, Issue 4, pp. 610-6, 2016 (PubMed).

de Oliveira, Reimer, Reis, Brandão: "Dopamine D2-like receptors modulate freezing response, but not the activation of HPA axis, during the expression of conditioned fear." dans: Experimental brain research, Vol. 235, Issue 2, pp. 429-436, 2016 (PubMed).

Zhao, Shin, Park, Yi, Lee, Lee: "Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Mice." dans: Neurochemical research, Vol. 42, Issue 4, pp. 1123-1129, 2016 (PubMed).

Wu, Song, Liu, Xing, Wang, Fei, Li, Zhang, Li, Zhang: "Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress." dans: Neuropeptides, Vol. 51, pp. 43-54, 2015 (PubMed).

Reimer, de Oliveira, Diniz, Hoexter, Chiavegatto, Brandão: "Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviors." dans: Behavioural brain research, Vol. 292, pp. 370-80, 2015 (PubMed).

Zhao, Shin, Choi, Lee: "Ameliorating effects of gypenosides on chronic stress-induced anxiety disorders in mice." dans: BMC complementary and alternative medicine, Vol. 15, pp. 323, 2015 (PubMed).

Chen, Li, Xia: "Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats." dans: International journal of clinical and experimental medicine, Vol. 8, Issue 7, pp. 11347-51, 2015 (PubMed).

Liu, Dong, Wang, Cao, Chen: "Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation." dans: Stress (Amsterdam, Netherlands), Vol. 17, Issue 6, pp. 494-503, 2014 (PubMed).

Habara, Hayashi, Inomata, Niijima, Kangawa: "Organ-specific activation of the gastric branch of the efferent vagus nerve by ghrelin in urethane-anesthetized rats." dans: Journal of pharmacological sciences, Vol. 124, Issue 1, pp. 31-9, 2014 (PubMed).

Pereiro, Moyano, Blanco, Lafuente: "Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic-pituitary-adrenal axis in adult male rats." dans: Toxicology letters, Vol. 230, Issue 2, pp. 252-62, 2014 (PubMed).