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HGF Kit ELISA

HGF Reactivité: Souris Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - citrate), Serum
N° du produit ABIN6719728
  • Antigène Voir toutes HGF Kits ELISA
    HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
    Reactivité
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - citrate), Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepapoietin A, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A scatter factor, Hepatocyte growth factor beta chain, Hepatopoietin-A, Hgf, HGF_HUMAN, HGFB, HPTA, Lung fibroblast derived mitogen, Scatter factor, SF

    Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

    Gene Name: HGF

    Production: Natural and recombinant mouse HGF. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: Q33-R495 (α)&V496-L728 (β)

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    Discover our top product HGF Kit ELISA
  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. We recommend that each mouse HGF standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 62.5pg/mL-4000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes HGF Kits ELISA
    HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
    Autre désignation
    HGF (HGF Produits)
    Synonymes
    DFNB39 Kit ELISA, F-TCF Kit ELISA, HGFB Kit ELISA, HPTA Kit ELISA, SF Kit ELISA, HGF Kit ELISA, C230052L06Rik Kit ELISA, HGF/SF Kit ELISA, NK1 Kit ELISA, NK2 Kit ELISA, SF/HGF Kit ELISA, hepatocyte growth factor Kit ELISA, HGF Kit ELISA, Hgf Kit ELISA
    ID gène
    15234
    NCBI Accession
    NP_001276387
    UniProt
    Q08048
    Pathways
    Signalisation RTK, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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