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FAS Kit ELISA

FAS Reactivité: Souris Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
N° du produit ABIN6719757
  • Antigène Voir toutes FAS Kits ELISA
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Reactivité
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: ALPS1A, APO 1, APO 1 cell surface antigen, Apo-1 antigen, APO1, Apo1 antigen, Apoptosis antigen 1, Apoptosis mediating surface antigen FAS, Apoptosis-mediating surface antigen FAS, APT1, CD95, CD95 antigen, Fas, Fas AMA, Fas antigen, FAS1, FASLG receptor, FASTM, TNF receptor superfamily member 6, TNFRSF6, TNR6_HUMAN, Tumor necrosis factor receptor superfamily member 6

    Background: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 419 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48- kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.

    Gene Name: FAS

    Production: Natural and recombinant mouse FAS. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: sf21, Immunogen sequence: Q22-R169

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FAS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each mouse FAS standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes FAS Kits ELISA
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Autre désignation
    FAS (FAS Produits)
    Synonymes
    ALPS1A Kit ELISA, APO-1 Kit ELISA, APT1 Kit ELISA, CD95 Kit ELISA, FAS1 Kit ELISA, FASTM Kit ELISA, TNFRSF6 Kit ELISA, AI196731 Kit ELISA, APO1 Kit ELISA, TNFR6 Kit ELISA, Tnfrsf6 Kit ELISA, lpr Kit ELISA, Fas cell surface death receptor Kit ELISA, Fas (TNF receptor superfamily member 6) Kit ELISA, FAS Kit ELISA, Fas Kit ELISA, fas Kit ELISA
    ID gène
    14102
    NCBI Accession
    NP_001140180
    UniProt
    Q8C350
    Pathways
    Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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