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CD86 Kit ELISA

CD86 Reactivité: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
N° du produit ABIN6719859
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    CD86
    Reactivité
    • 6
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    • 3
    • 1
    • 1
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    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: Activation B7 2 antigen, B7 2, B70, B72, BU63, CD28LG2, CD86 antigen (CD28 antigen ligand 2 B7 2 antigen), CD86 Molecule, CTLA 4 counter receptor B7.2, FUN 1, FUN1, T lymphocyte activation antigen CD86 precursor

    Background: Cluster of Differentiation 86(also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

    Gene Name: CD86

    Production: Natural and recombinant rat CD86. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: V29-I250

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD86 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. It is recommended that each rat CD86 standard solution and each sample be measured in duplicate.

    ELISA Dilution: 62.5pg/mL-4000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes CD86 Kits ELISA
    CD86
    Autre désignation
    CD86 - B7-2 (CD86 Produits)
    Synonymes
    B7-2 Kit ELISA, B7.2 Kit ELISA, B70 Kit ELISA, CD28LG2 Kit ELISA, LAB72 Kit ELISA, B7 Kit ELISA, CLS1 Kit ELISA, Cd28l2 Kit ELISA, ETC-1 Kit ELISA, Ly-58 Kit ELISA, Ly58 Kit ELISA, MB7 Kit ELISA, MB7-2 Kit ELISA, TS/A-2 Kit ELISA, CD86 molecule Kit ELISA, CD86 antigen Kit ELISA, integrin subunit alpha 2b Kit ELISA, CD86 Kit ELISA, Cd86 Kit ELISA, Itga2b Kit ELISA
    ID gène
    56822
    NCBI Accession
    NP_064466
    UniProt
    Q62810
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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