PCSK9 Kit ELISA

N° du produit ABIN6720072

Détail du produit PCSK9 Kit ELISA

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Convertase subtilisin/kexin type 9 preproprotein, FH3, HCHOLA3, Hypercholesterolemia autosomal dominant 3, LDLCQ1, NARC 1, NARC-1, NARC1, Neural apoptosis regulated convertase 1, Neural apoptosis-regulated convertase 1, PC 9, PC9, PCSK 9, PCSK9, PCSK9_HUMAN, Proprotein convertase 9, Proprotein convertase PC9, Proprotein convertase subtilisin/kexin type 9, PSEC0052, Subtilisin/kexin like protease PC9, Subtilisin/kexin-like protease PC9

Background: Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.

Gene Name: PCSK9

Production: Natural and recombinant human PCSK9. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S153-Q692

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PCSK9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human PCSK9 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Autre désignation: PCSK9 (PCSK9 Produits)

Synonymes: FH3 Kit ELISA, HCHOLA3 Kit ELISA, LDLCQ1 Kit ELISA, NARC-1 Kit ELISA, NARC1 Kit ELISA, PC9 Kit ELISA, AI415265 Kit ELISA, AI747682 Kit ELISA, Narc1 Kit ELISA, proprotein convertase subtilisin/kexin type 9 Kit ELISA, proprotein convertase subtilisin/kexin type 9 L homeolog Kit ELISA, PCSK9 Kit ELISA, pcsk9.L Kit ELISA, pcsk9 Kit ELISA, Pcsk9 Kit ELISA

ID gène: 255738

NCBI Accession: NP_777596

UniProt: Q8NBP7