NBL1 Kit ELISA

N° du produit ABIN6720249

Détail du produit NBL1 Kit ELISA

Antigène: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: D1S1733E, DAN, DAN domain family member 1, DAND 1, DAND1, Differential screening selected gene aberrant in neuroblastoma, MGC8972, NB, NBL 1, Neuroblastoma candidate region suppression of tumorigenicity 1, Neuroblastoma suppression of tumorigenicity 1, Neuroblastoma suppressor of tumorigenicity 1, NO 3, NO3, Zinc finger protein DAN

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Gene Name: NBL1

Production: Natural and recombinant mouse DAN. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A17-D178

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse DAN standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Autre désignation: DAN - NBL1 (NBL1 Produits)

Synonymes: D1S1733E Kit ELISA, DAN Kit ELISA, DAND1 Kit ELISA, NB Kit ELISA, NO3 Kit ELISA, D4H1S1733E Kit ELISA, Dana Kit ELISA, dand1 Kit ELISA, nbl1-a Kit ELISA, no3 Kit ELISA, 4.1N Kit ELISA, Epb41l1 Kit ELISA, NBL1 Kit ELISA, mKIAA0338 Kit ELISA, C1orf151-NBL1 Kit ELISA, dan Kit ELISA, zgc:77251 Kit ELISA, neuroblastoma 1, DAN family BMP antagonist Kit ELISA, neuroblastoma, suppression of tumorigenicity 1 Kit ELISA, neuroblastoma 1, DAN family BMP antagonist L homeolog Kit ELISA, neuroblastoma suppressor of tumorigenicity 1 Kit ELISA, erythrocyte membrane protein band 4.1 like 1 Kit ELISA, MINOS1-NBL1 readthrough Kit ELISA, NBL1 Kit ELISA, Nbl1 Kit ELISA, nbl1.L Kit ELISA, LOC535280 Kit ELISA, Epb41l1 Kit ELISA, MINOS1-NBL1 Kit ELISA, nbl1 Kit ELISA

ID gène: 17965

NCBI Accession: NP_032701

UniProt: Q61477