ANG Kit ELISA

N° du produit ABIN6720265

Détail du produit ANG Kit ELISA

Antigène: ANG (Angiogenin (ANG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: ALS9, ANG, ANG 1, ANG I, ANG1, ANGI, Angiogenin ribonuclease RNase A family 5, Epididymis luminal protein 168, HEL168, MGC22466, MGC71966, Ribonuclease 5, Ribonuclease RNase A Family 5, Ribonuclease5, RNASE 5, RNASE4, RNASE5

Background: Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Gene Name: ANG

Production: Natural and recombinant human ANG. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: Q25-P147

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human ANG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANG standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: ANG (Angiogenin (ANG))

Autre désignation: ANG (ANG Produits)

Synonymes: ALS9 Kit ELISA, HEL168 Kit ELISA, RNASE4 Kit ELISA, RNASE5 Kit ELISA, ANG Kit ELISA, AI385586 Kit ELISA, Ang1 Kit ELISA, Rnase5 Kit ELISA, Rnase5a Kit ELISA, ANG1 Kit ELISA, angiogenin Kit ELISA, angiogenin, ribonuclease, RNase A family, 5 Kit ELISA, ribonuclease A family member k6 Kit ELISA, ANG Kit ELISA, Ang Kit ELISA, RNASE6 Kit ELISA

ID gène: 283

NCBI Accession: NP_001091046

UniProt: P03950