FCN1 Kit ELISA

N° du produit ABIN6720297

Détail du produit FCN1 Kit ELISA

Antigène: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Collagen/fibrinogen domain containing protein 1, Collagen/fibrinogen domain-containing protein 1, Fcn1, FCN1_HUMAN, FCNM, ficolin (collagen/fibrinogen domain containing) 1, Ficolin 1, Ficolin A, Ficolin alpha, Ficolin-1, Ficolin-A, Ficolin-alpha, M ficolin, M-ficolin

Background: Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Gene Name: FCN1

Production: Natural and recombinant human Ficolin-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A30-A326

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Ficolin-1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Autre désignation: Ficolin-1 (FCN1 Produits)

Synonymes: FCNM Kit ELISA, FCNB Kit ELISA, FCN2 Kit ELISA, FCN1 Kit ELISA, ficolin 1 Kit ELISA, ficolin (collagen/fibrinogen domain containing) 1 Kit ELISA, ficolin-1 Kit ELISA, FCN1 Kit ELISA, Fcn1 Kit ELISA, LOC100069029 Kit ELISA

ID gène: 2219

NCBI Accession: NP_001994

UniProt: O00602

Pathways: Système du Complément