FCN3 Kit ELISA
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- Antigène Voir toutes FCN3 Kits ELISA
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.78 ng/mL - 50 ng/mL
- Seuil minimal de détection
- 0.78 ng/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: Collagen/fibrinogen domain containing lectin 3 p35, Collagen/fibrinogen domain containing protein 3, FCN 3, FCNH, Ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen), Ficolin (collagen/fibrinogen domain containing) 3, Ficolin 3, H ficolin, HAKA1, Hakata antigen, MGC22543
Background: Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin(FN1), elastin(ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.
Gene Name: FCN3
Production: Natural and recombinant human Ficolin-3. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: Q24-R299
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- Discover our top product FCN3 Kit ELISA
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Ficolin-3 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 0.78 ng/mL-50 ng/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes FCN3 Kits ELISA
- FCN3 (Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3))
- Autre désignation
- Ficolin-3 (FCN3 Produits)
- Synonymes
- FCNH Kit ELISA, HAKA1 Kit ELISA, ficolin 3 Kit ELISA, ficolin-3 Kit ELISA, FCN3 Kit ELISA, Fcn3 Kit ELISA, fcn3-A Kit ELISA
- ID gène
- 8547
- NCBI Accession
- NP_003656
- UniProt
- O75636
- Pathways
- Système du Complément
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