E-cadherin Kit ELISA
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- Antigène Voir toutes E-cadherin (CDH1) Kits ELISA
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Plasma (heparin), Serum
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: Arc 1, Cadherin 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD 234, CD324, CD324 antigen, CDH1, CDHE, ECAD, Epithelial cadherin, epithelial calcium dependant adhesion protein, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin
Background: Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
Gene Name: E-Cadherin
Production: Natural and recombinant rat E-Cadherin. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: Q24-S704
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each rat E-Cadherin standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes E-cadherin (CDH1) Kits ELISA
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- Autre désignation
- E-Cadherin (CDH1 Produits)
- Synonymes
- Arc-1 Kit ELISA, CD324 Kit ELISA, CDHE Kit ELISA, ECAD Kit ELISA, LCAM Kit ELISA, UVO Kit ELISA, E-cadherin Kit ELISA, cdh1 Kit ELISA, si:dz180o5.2 Kit ELISA, uvo Kit ELISA, lcam Kit ELISA, E-Cad Kit ELISA, XBcad Kit ELISA, l-cam Kit ELISA, xcdh1 Kit ELISA, cdhc-A Kit ELISA, xb-cad Kit ELISA, XTCAD-1 Kit ELISA, c-cadherin Kit ELISA, cadherin-1 Kit ELISA, uvomorulin Kit ELISA, XB-cadherin Kit ELISA, arc-1 Kit ELISA, cd324 Kit ELISA, cdhe Kit ELISA, ecad Kit ELISA, AA960649 Kit ELISA, E-cad Kit ELISA, Ecad Kit ELISA, L-CAM Kit ELISA, Um Kit ELISA, cadherin 1 Kit ELISA, si:busm1-180o5.2 Kit ELISA, cadherin 1, type 1, E-cadherin (epithelial) Kit ELISA, cadherin 1, type 1 S homeolog Kit ELISA, CDH1 Kit ELISA, si:busm1-180o5.2 Kit ELISA, cdh1 Kit ELISA, Cdh1 Kit ELISA, cdh1.S Kit ELISA
- ID gène
- 83502
- NCBI Accession
- NP_112624
- UniProt
- Q9R0T4
- Pathways
- Signalisation WNT, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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